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C. M. Saporito-Magrina, R. N. Musacco-Sebio, G. Andrieux, L. Kook, M. T. Orrego, M. V. Tuttolomondo, M. F. Desimone, M. Boerries, C. Borner and M. G. Repetto, Metallomics, 2018, 10, 1743–1754 CrossRef CAS PubMed.

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The R21/Matrix-M™ malaria vaccine, developed by the University of Oxford, leveraging an adjuvant technology from Novavax, and manufactured and scaled up by the SIIPL, has been granted full national licensure for use in Ghana by the country’s Food and Drugs Authority (FDA Ghana).

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Malaria is a life-threatening disease that disproportionately affects the most vulnerable populations in our society and remains a leading cause of death in childhood. Developing a vaccine to greatly impact this huge disease burden has been extraordinarily difficult. At the Serum Institute of India, we are committed to our vision of Health for All and ensuring equitable access to vaccines for people around the world. The Matrix-M component is a proprietary saponin-based adjuvant from Novavax, which is licensed to the Serum Institute for use in endemic countries, while Novavax retains commercial rights in non-endemic countries. Richter, K. R., Nasr, A. N. & Mexas, A. M. Cytokine concentrations measured by multiplex assays in canine peripheral blood samples. Vet. Pathol. 55(1), 53–67 (2018). Swart DJ, Simeonsson JB. Development of an electrothermal atomization laser-excited atomic fluorescence spectrometry procedure for direct measurements of arsenic in diluted serum. Anal Chem. 1999;71(21):4951–5. Zhao S, Cao S, Luo L, Zhang Z, Yuan G, Zhang Y, Yang Y, Guo W, Wang L, Chen F, et al. A preliminary investigation of metal element profiles in the serum of patients with bloodstream infections using inductively-coupled plasma mass spectrometry (ICP-MS). Clin Chim Acta. 2018;485:323–32.

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Linearity (measured as observed concentration relative to the expected concentration in a sample with known dilution) was evaluated using the same neat serum pool samples used in for the evaluation of assay recovery. Each sample was spiked with the highest concentration kit standard and then measured at 1:2 and 1:5 assay buffer dilutions. Three of the eight samples were also measured neat (unspiked) and at a 1:2 dilution (Supplemental Table 2). Each sample was tested in triplicate. PrecisionC. D. Palmer, M. E. Lewis Jr, C. M. Geraghty, F. Barbosa Jr and P. J. Parsons, Spectrochim. Acta, Part B, 2006, 61, 980–990 CrossRef. Multiplexing technology has significant animal welfare and research advantages when compared to traditional methods like ELISA; the ability to simultaneously measure multiple cytokines from a single small volume sample allows for reduced animal handling, reduced blood draw volume, and increased research efficiency. While multiplex assays cost more than individual ELISAs, they are relatively cost-effective when four or more analytes can be accurately measured 46. Furthermore, the need to evaluate multiple cytokine levels together to better understand the overall clinical picture of a subject makes multiplexing an attractive method. Comparisons of multiplex-based immunoassays with single analyte ELISAs showed that while measurements of high abundance cytokines were highly correlated between methodologies, low abundance cytokines in healthy human subjects (IL-6, TNFα, IL-1β) were poorly correlated 47. This trade-off between multiplexing and assay sensitivity for low abundance cytokines must be carefully considered when designing a study, and caution should be used when interpreting results near the LLOD of a multiplex assay. The popularity of multiplex assays and the importance of NHPs in pre-clinical testing has resulted in an increase in the number of NHP multiplex cytokine assays on the market. Given the importance of understanding the cytokine response in pre-clinical testing, it will be critical to continue validation efforts of these commonly used assays.

A simple, rapid method for simultaneous determination of

Oxford is world-famous for research and teaching excellence and home to some of the most talented people from across the globe. Our work helps the lives of millions, solving real-world problems through a huge network of partnerships and collaborations. The breadth and interdisciplinary nature of our research alongside our personalised approach to teaching sparks imaginative and inventive insights and solutions.N. Laur, R. Kinscherf, K. Pomytkin, L. Kaiser, O. Knes and H.-P. Deigner, PLoS One, 2020, 15, e0233357 CrossRef CAS PubMed. F. Barbosa Jr, J. E. Tanus-Santos, R. F. Gerlach and P. J. Parsons, Environ. Health Perspect., 2005, 113, 1669–1674 CrossRef PubMed. We are very pleased to see the enthusiasm with which the communities of Bougouni received the first doses of this new candidate malaria vaccine. We are hopeful that the encouraging Phase 2 results will be confirmed in this larger Phase 3 trial'. Toxicological profile for cadmium, U.S. Department of Heath and Human Services, Public Health Service, https://www.atsdr.cdc.gov/toxprofiles/tp5.pdf, accessed November 11, 2021 Search PubMed. J.-P. Goulle, P. L. Roux, M. Castanet, L. Mahieu, S. Guyet-Job and M. Guerbet, J. Anal. Toxicol., 2015, 39, 707–713 CrossRef CAS PubMed.

Serum and Plasma Matrices on the Titration of Human Impact of Serum and Plasma Matrices on the Titration of Human

Alimonti A, Bocca B, Mannella E, Petrucci F, Zennaro F, Cotichini R, D’Ippolito C, Agresti A, Caimi S, Forte G. Assessment of reference values for selected elements in a healthy urban population. Ann Ist Super Sanita. 2005;41(2):181–7. Chaudhry, H. et al. Role of cytokines as a double-edged sword in sepsis. In Vivo. 27(6), 669–684 (2013) (Epub 2013/12/03). Cytokines transmit intracellular signals that are central to immune homeostasis or dysregulation and have a central role in pathologies like obesity and diabetes or cancer and autoimmunity. Conversely, the normal cytokine response can also be a target for immune manipulation like in the situation of transplantation where the host immune response to the graft must be suppressed to prevent rejection. In transplantation the achievement of immune tolerance reduces the risk of rejection and improves safety by eliminating toxic immunosuppression 6. Immune profiling cytokines in health and disease has led to the development of numerous immunotherapy medications, both agonists and antagonists, for many diseases such as adult and juvenile rheumatoid arthritis, psoriasis, Inflammatory Bowel Disease, melanoma, colon cancer, lung cancer, renal cell carcinoma, multiple myeloma, and AIDS. For example, Adalimumab, an anti-TNFα antibody, has been used successfully to treat Inflammatory Bowel Disease 7. IL-2 and IFNα in combination with chemotherapy have been used to treat metastatic melanoma, but development of many other cytokine treatments like IL-15 as single agents has not proceeded beyond Phase 2 trials 8. Clinical inefficacy or negative side effects have so far limited the wide clinical success of cytokine-based therapies 9, 10, which is related to cytokine signaling across multiple pathways and many that exhibit redundancy and pleiotropy. The complexity of these targets drives the need for accurate and reliable measurements of cytokines to continue to advance the understanding of immune pathophysiology and immunomodulation strategies. I. D. B. Bravo, R. S. Castro, N. L. Riquelme, C. T. Diaz and D. A. Goyenaga, J. Trace Elem. Med. Biol., 2007, 21, 14–17 CrossRef PubMed. Heitland P, Köster HD. Human biomonitoring of 73 elements in blood, serum, erythrocytes and urine. J Trace Elem Med Biol. 2021;64: 126706.Toxicological profile for mercury, U.S. Department of Heath and Human Services, Public Health Service, https://www.atsdr.cdc.gov/toxprofiles/tp46.pdf, accessed November 11, 2021 Search PubMed. M. d. Oliveira, T. M. R. Gianeti, F. C. G. d. Rocha, P. N. Lisboa-Filho and M. Piacenti-Silva, Sci. Rep., 2020, 10, 13112 CrossRef PubMed. Didier, E. S. et al. Contributions of nonhuman primates to research on aging. Vet. Pathol. 53(2), 277–290. https://doi.org/10.1177/0300985815622974 (2016) (Epub 2016/02/13). The R21/Matrix-M™ vaccine is licensed to the Serum Institute of India, the world’s largest vaccine manufacturer as well as a long-term partner of Oxford University. Notably, the Serum Institute has already established production capacity for 100 million doses per annum, which will be doubled over the next two years. This scale of production is critical because vaccinating those at high risk of malaria will be important in stemming the spread of disease, as well as protecting the vaccinated.